Jakob Prömer
PhD- StudentDepartment(s): Institute of Specific Prophylaxis and Tropical Medicine (Center for Pathophysiology, Infectiology and Immunology)
Position: PHD Student
Location: Kinderspitalgasse 15
E-Mail: jakob.proemer@meduniwien.ac.at
Abstract:
The neuromuscular junction connects nervous system and muscle tissue. Muscle specific kinase (MuSK) is a receptor tyrosine kinase that is crucial for its formation. I aim to characterize a specific serine residue in MuSK that relates to its activation. I will identify the kinase that phosphorylates this serine, and analyse the kinase domains of wildtype- and phosphomimetic mutant MuSK in a structure-function approach. A transgenic mouse model will provide understanding of the effect of serine phosphorylation in vivo. These data will provide insight in the mechanism of MuSK activation and potential rescue mechanisms for the development of the neuromuscular junction.
Techniques:
Cell culture
Western Blot
Molecular Cloning
Fast Protein Liquid Chormatography
Histology and Immunocytochemistry
Fluorescence- and confocal laser scanning microscopy
Selected Publications:
- Prömer J, Sombke A, Schwaha T (2021) A comparative analysis of the nervous system of cheilostome bryozoans BMC, 6 (1)